Dengue Fever Rapid Test Kits or devices, also known as one-step Dengue tests, are a solid phase immuno-chromatographic assay for the rapid, qualitative and differential detection of Dengue IgG and IgM antibodies to dengue fever virus in human serum, plasma or whole blood. This is a common test used to detect presence of dengue antibodies in the blood of a suspected dengue case.
How Does it Work?
- Once the sample is added to the test cassette along with the diluents, the mixture passes through the antibody binding/gold complex, which then binds the immunoglobulins in the sample.
- As this complex passes over the immobilized antigens on the membrane, if any antibodies to Dengue are present, the antigens capture them.
- In turn, this produces a pink/purple band in the T (test) zone of the test strip.
- The remaining complex continues to migrate to a control area in the test strip and produces a pink/purple band in the C (controlled) area.
- This band indicates that the test has been performed properly.
Components of the Kit
- Test cassette
- Diluent in dropper vial
- Directions for use
- Dengue test kit is stable at any room temperature between 8-30 oC when in the unopened pouches.
- Do not freeze the kit or expose to extreme temperatures
- Stable until 24 months from the date of manufacture
- Test is for In Vitro Diagnostic use only
- Appropriate infection control measures should be followed
- All materials should be considered infectious and must be discarded or disinfected properly.
- Do not use test beyond the expiration date indicated.
- Dengue IgG/IgM test can be run on serum or whole blood.
- The test works best on fresh samples.
- If testing cannot be done immediately, samples should be stored at 2-8 C after collection, and may remain in storage for up to 3 days.
- If testing cannot be done within 3 days, serum can be stored frozen at -20C or colder.
- Whole blood samples cannot be frozen, nor samples collected in EDTA, and it is recommended that finger prick blood be used.
- Plasma may be used depended on anticoagulant.
- Remove test cassettes from the pouches as needed. Lay on a clean flat surface.
- For whole blood–add 10micro of sample to Sample Well labeled A of the test cassette using measuringpipette.
For serum–add 5micro sample to the sample well labeled A of the test casette.
- Follow sample addition with 3 drops of the diluent provided in the dropper bottle by holding the bottle vertically over the (S) well.
- Results are then read in after 5 minutes for strong positives, or up to 30 minutes for weaker positives and to make sure negatives are confirmed.
Note: If the dye has not cleared the membrane, or blood is still present, one or two more drops of diluents may be added
to the (S) well.
Reading the Results
Only 1 (one) pink/purple band appears in the C (control) line area of the test casette.
Two pink/purple bands appear, one in the C (control line), and one in the T (test line) area of the test strip.
If only one band appears in the T well – Test area, or no band appears at all in the C well –Control area, it is then recommended that a fresh device be used and the test repeated, carefully following the directions in this insert. Test should be repeated.
- A clinical evaluation of patient’s signs and symptoms should be established and correlated with the test resultsbefore making a final diagnosis.
- Using rapid test alone is not sufficient to diagnose Dengue fever even if antibodies are present. Also, a negative results does not preclude the possibility of infection with Dengue virus.
- Observe proper hand hygiene
- Infection control measures should be observed
- Establish baseline data, obtain a nursing assessment
- Prompt referral
References & Sources
- Health Chem Diagnostics
- Dengue Fever Rapid Test Kit